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See <a href='https://www.quantics.co.uk/blog/when-is-pure-error-for-bioassay-an-error/' target='_blank'>blog</a> on Quantics website for explanation.","description-analysisdef-linearpartidentification":"QuBAS can progressively remove points from the top, bottom, or both ends of a data set to achieve linearity. <span class=\"description-red\">Critical p value recommendation p=0.01</span>. The point removal option is applied to both the reference and samples.","description-analysisdef-meanofgroup":"Control wells are groups of wells that have the same dose. Suitability criteria for the arithmetic mean of the responses can be set for all control wells (global setting) or for individual groups of wells if they are separately defined in the data map.","description-analysisdef-totalofgroup":"Control wells are groups of wells that have the same dose. Suitability criteria for the total number of responses can be set for all control wells (global setting) or for individual groups of wells if they are separately defined in the data map.","description-analysisdef-geometricmean":"Control wells are groups of wells that have the same dose. Suitability criteria for the geometric mean of the responses can be set for all control wells (global setting) or for individual groups of wells if they are separately defined in the data map.","description-analysisdef-goodnessoffit":"If there is more than one replicate at each dose lack of fit error is compared with pure error via an F test. <span class=\"description-red\">Recommended critical value p=0.01.</span><br/>If there is only one replicate a quadratic model is fitted to the data and the significance of the quadratic term tested via a t test. <span class=\"description-red\">Recommended critical value p=0.01.</span><br/><span class=\"description-red\">Caution:</span> Goodness of fit tests may inappropriately fail modern, very precise, assays. Consider other tests such as variability instead. Please contact Quantics for more help on this.","description-analysisdef-rsquared":"The r² for the regression can be calculated. Enter minimum acceptable value.","description-analysisdef-rangeintercept":"Set criteria for the acceptable range of the intercept value.","description-analysisdef-rangeslope":"Set criteria for the acceptable range of the slope.","description-analysisdef-equivalenceintercept":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval of the intercept value. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-equivalenceslope":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval of the slope. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-significanceofslope":"Tests whether the slope is significantly different from zero. <span class=\"description-red\">Recommended critical value = 0.01.</span>","description-analysisdef-variability":"Set limits based on historical data.","description-analysisdef-rangeslopedifference":"Set acceptable range for the difference between the Reference and QC sample slopes based on historical data.","description-analysisdef-rangesloperatio":"Set acceptable range for the ratio between the Reference and QC sample slopes based on historical data.","description-analysisdef-rangenormalisedslopedifference":"Set acceptable range for the difference between the Reference and QC sample slopes divided by the average of the slopes.","description-analysisdef-equivalenceslopedifference":"Set acceptable range for the confidence interval of the difference between the Reference and QC sample slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-equivalencesloperatio":"Set acceptable range for the confidence interval of the ratio of the Reference and QC sample slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-equivalencenormalisedslopedifference":"Set acceptable range for the confidence interval of the difference between the Reference and QC sample slopes divided by the average of the slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90</span>","description-analysisdef-parallelism":"F test and χ² test compare the model with separate slopes with the parallel model. <span class=\"description-red\">Recommended p=0.01.</span><br/>The χ² test should be used if weightings have been selected to normalise the variance. If not, then use F test.","description-analysisdef-rangerelativepotency":"Set acceptable range for the RP of the QC sample.","description-analysisdef-rangeresult":"Set acceptable range for the Result of the QC sample.","description-analysisdef-equivalencerelativepotency":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval of the QC RP.","description-analysisdef-equivalenceresult":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval of the QC Result.","description-analysisdef-foldprecision":"Set a limit on the ratio of the upper CL / lower CL for the RP.","description-analysisdef-rangeslopedifferencesample":"Set acceptable range for the difference between the Reference and sample slopes based on historical data.","description-analysisdef-rangesloperatiosample":"Set acceptable range for the ratio between the Reference and sample slopes based on historical data.","description-analysisdef-rangenormalisedslopedifferencesample":"Set acceptable range for the difference between the Reference and sample slopes divided by the average of the slopes.","description-analysisdef-equivalenceslopedifferencesample":"Set acceptable range for the confidence interval of the difference between the Reference and sample slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-equivalencesloperatiosample":"Set acceptable range for the confidence interval of the ratio of the Reference and sample slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-equivalencenormalisedslopedifferencesample":"Set acceptable range for the confidence interval of the difference between the Reference and sample slopes divided by the average of the slopes based on historical data. <span class=\"description-red\">Recommended confidence  level = 0.90</span>","description-analysisdef-bispositive":"The B parameter is related to the slope. However, the 4pl equation can be set up in various ways. If you want to compare QuBAS 4pl fits with historical analyses outside QuBAS then select whether you expect the B parameter to be positive or negative. The fit will be the same, but the values assigned to the parameters A, B, D will swap round.","description-analysisdef-methodforratios4pl5pl":"There are two methods commonly used to calculate the confidence intervals for ratio results such as the slope ratio. Fieller's theorem is specifically for ratios but can sometimes create nonsensical intervals. The delta method is more generally applicable and gives symmetric confidence intervals. The results in most datasets are very similar.","description-analysisdef-goodnessoffit4pl":"If there is more than one replicate at each dose lack of fit error is compared with pure error via an F test. <span class=\"description-red\">Recommended critical value p=0.01.</span><br/>If there is only one replicate a 5PL model is fitted to the data and the significance of the asymmetry term tested via a Chi-squared test. <span class=\"description-red\">Recommended critical value p=0.01.</span><br/><span class=\"description-red\">Caution:</span> Goodness of fit tests may inappropriately fail modern, very precise, assays. Consider other tests such as variability instead. Please contact Quantics for more help on this.","description-analysisdef-rangefora":"Set criteria for the acceptable range of A. This is the left asymptote if B is positive and the right asymptote if B is negative.","description-analysisdef-rangeforb":"Set criteria for the acceptable range of the slope parameter.","description-analysisdef-rangeforc":"Set criteria for the acceptable range of the location parameter.","description-analysisdef-rangeford":"Set criteria for the acceptable range of D. This is the right asymptote if B is positive and the left asymptote if B is negative.","description-analysisdef-confidenceintervalfora":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for A. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforb":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for B. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforc":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for C. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalford":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for D. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-significanceofdoseresponse":"Tests whether the slope parameter is significantly different from zero. <span class=\"description-red\">Recommended critical value =  0.01.</span>","description-analysisdef-range":"Set criteria (\"gateposts\") for the reference range = |D-A|.","description-analysisdef-confidenceintervalforrange":"Set criteria (\"gateposts\") for the confidence interval of the reference range =|D-A|. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-rangeforadifference":"Set acceptable range for the difference between the Reference and QC A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangeforddifference":"Set acceptable range for the difference between the Reference and QC D parameters (right asymptotes if B is positive and left asymptotes if B is negative).","description-analysisdef-rangefornormalisedadifference":"Set acceptable range for the difference between the Reference and QC A parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangefornormalisedddifference":"Set acceptable range for the difference between the Reference and QC D parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangefornormalisedbdifference":"Set acceptable range for the difference between the Reference and QC B parameters divided by the mean of the B parameters (Bref + B)/2.","description-analysisdef-rangeforbdifference":"Set acceptable range for the difference between the Reference and QC B parameters.","description-analysisdef-rangeforsloperatio":"Set limits for the acceptable range of the ratio of QC sample and reference B parameters.","description-analysisdef-confidenceintervalforadifference":"Set acceptable range for the confidence interval of the difference between the Reference and QC sample A parameters (left asymptotes if B is positive and right asymptotes if B is negative). <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforddifference":"Set acceptable range for the confidence interval of the difference between the Reference and QC sample D parameters (left asymptotes if B is positive and right asymptotes if B is negative). <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedadifference":"Set acceptable range for the confidence interval of (difference between the Reference and QC sample A parameters / reference range). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedddifference":"Set acceptable range for the confidence interval of (difference between the Reference and QC sample D parameters / reference range). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedbdifference":"Set acceptable range for the confidence interval of (difference between the Reference and QC B parameters / mean of the B parameters). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforbdifference":"Set acceptable range for the confidence interval of difference between the Reference and QC sample slope parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforsloperatio":"Set acceptable range for the confidence interval of the ratio between the QC sample and Reference slope parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-parallelism4pl5pl":"F test and χ² test compare  the model with separate A, B and D parameters with the parallel model. <span class=\"description-red\">Recommended p=0.01.</span><br/>The χ² test should be used if weightings have been selected to normalise the variance. If not, then use F test.","description-analysisdef-rangeforadifferencesample":"Set acceptable range for the difference between the Reference and sample A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangeforddifferencesample":"Set acceptable range for the difference between the Reference and sample D parameters (right asymptotes if B is positive and left asymptotes if B is negative).","description-analysisdef-rangefornormalisedadifferencesample":"Set acceptable range for the difference between the Reference and sample A parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangefornormalisedddifferencesample":"Set acceptable range for the difference between the Reference and sample D parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangefornormalisedbdifferencesample":"Set acceptable range for the difference between the Reference and sample B parameters divided by the mean of the B parameters (Bref + B)/2.","description-analysisdef-rangeforbdifferencesample":"Set acceptable range for the difference between the Reference and sample B parameters.","description-analysisdef-rangeforsloperatiosample":"Set limits for the acceptable range of the ratio of sample and reference B parameters.","description-analysisdef-confidenceintervalforadifferencesample":"Set acceptable range for the confidence interval of the difference between the Reference and sample A parameters (left asymptotes if B is positive and right asymptotes if B is negative). <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforddifferencesample":"Set acceptable range for the confidence interval of the difference between the Reference and sample D parameters (left asymptotes if B is positive and right asymptotes if B is negative). <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedadifferencesample":"Set acceptable range for the confidence interval of (difference between the Reference and sample A parameters / reference range). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedddifferencesample":"Set acceptable range for the confidence interval of (difference between the Reference and sample D parameters / reference range). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalfornormalisedbdifferencesample":"Set acceptable range for the confidence interval of (difference between the Reference and sample B parameters / mean of the B parameters). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforbdifferencesample":"Set acceptable range for the confidence interval of difference between the Reference and sample slope parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforsloperatiosample":"Set acceptable range for the confidence interval of the ratio between the sample and Reference slope parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-bsign":"The B parameter is related to the slope, and whether it is positive or negative controls whether the A parameter is the left asymptote and the D parameter is the right asymptote or vice versa. The 5pl curve is asymmetric, so the fit changes depending on this choice. If 'Unrestricted' is selected, QuBAS will try both ways and choose the best.","description-analysisdef-goodnessoffit5pl":"If there is more than one replicate at each dose lack of fit error is compared with pure error via an F test. <span class=\"description-red\">Recommended critical value p=0.01.</span><br/>If there is only one replicate no formal goodness of fit test is available.<br/><span class=\"description-red\">Caution:</span> Goodness of fit tests may inappropriately fail modern, very precise, assays. Consider other tests such as variability instead. Please contact Quantics for more help on this.","description-analysisdef-rangefore":"Set criteria for the asymmetry parameter E.","description-analysisdef-locationofmidpoint":"Set criteria for the midpoint (ED<sub>50</sub>).","description-analysisdef-amountofassymetry":"Set criteria for the acceptable range of log(E). This is zero when the curve is symmetrical.","description-analysisdef-confidenceintervalfore":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for E. <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforlocationofmidpoint":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for the midpoint (ED₅₀). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-confidenceintervalforamountofassymetry":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval for log(E). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-rangeadifference":"Set acceptable range for the difference between the Reference and QC A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangeddifference":"Set acceptable range for the difference between the Reference and QC D parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangenormalisedadifference":"Set acceptable range for the difference between the Reference and QC A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangenormalisedddifference":"Set acceptable range for the difference between the Reference and QC D parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangebdifference":"Set acceptable range for the difference between the Reference and QC B parameters.","description-analysisdef-rangeedifference":"Set acceptable range for the difference between the Reference and QC E parameters.","description-analysisdef-rangeassymetrydifference":"Set acceptable range for the difference between log(E) for the Reference and QC.","description-analysisdef-rangesloperatio5pl":"Set limits for the acceptable range of the ratio of QC sample and reference B parameters.","description-analysisdef-confidenceintervalforedifference":"Set acceptable range for the confidence interval of the difference between the Reference and QC sample asymmetry parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforedifferencesample":"Set acceptable range for the confidence interval of the difference between the Reference and sample asymmetry parameters. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforassymetrydifference":"Set acceptable range for the confidence interval of the difference between log(E) for the Reference and QC. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-confidenceintervalforassymetrydifferencesample":"Set acceptable range for the confidence interval of the difference between log(E) for the Reference and sample. <span class=\"description-red\">Recommended confidence  level = 0.90.</span>","description-analysisdef-rangeadifferencesample":"Set acceptable range for the difference between the Reference and sample A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangeddifferencesample":"Set acceptable range for the difference between the Reference and sample D parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangenormalisedadifferencesample":"Set acceptable range for the difference between the Reference and sample A parameters (left asymptotes if B is positive and right asymptotes if B is negative).","description-analysisdef-rangenormalisedddifferencesample":"Set acceptable range for the difference between the Reference and sample D parameters divided by the reference range |Aref - Dref|.","description-analysisdef-rangebdifferencesample":"Set acceptable range for the difference between the Reference and sample B parameters.","description-analysisdef-rangeedifferencesample":"Set acceptable range for the difference between the Reference and sample E parameters.","description-analysisdef-rangeassymetrydifferencesample":"Set acceptable range for the difference between log(E) for the Reference and sample.","description-analysisdef-rangesloperatio5plsample":"Set limits for the acceptable range of the ratio of sample and reference B parameters.","description-analysisdef-goodnessoffitquantal":"A quadratic model is fitted to the data and the significance of the quadratic term tested via a z test. <span class=\"description-red\">Recommended critical value p=0.01</span>","description-analysisdef-parallelismquantal":"The χ² test compares the model with separate slopes with the parallel model. <span class=\"description-red\">Recommended p=0.01.</span>","description-analysisdef-rangemidpoint":"Set criteria for the acceptable range of the midpoint (= -A/B).","description-analysisdef-equivalencemidpoint":"Set criteria (\"gateposts\") for the acceptable range for the confidence interval of the midpoint (=-A/B). <span class=\"description-red\">Recommended confidence level = 0.90.</span>","description-analysisdef-parallelismia":"This is a goodness of fit test that checks the fit of the sample point to the best fit line that is also parallel to the reference. The test uses an approximation of the F test. If there is only one dilution then no test is possible. <span class=\"description-red\">Recommended p=0.01.</span>","description-analysisdef-parallelismia4pl5pl":"This is a goodness of fit test that checks the fit of the sample point to the best fit curve that is also parallel to the reference. The test uses an approximation of the F test. If there is only one dilution then no test is possible.  <span class=\"description-red\">Recommended p=0.01.</span>","description-analysisdef-variabilityia":"An upper limit can be set on the Variance or RMSE","description-analysisdef-foldprecisionia":"An upper limit can be set on the ratio: Upper confidence limit / lower confidence limit for the reportable value. Typical values < 3 but depend on precision requirement.","description-analysisdef-pseudoreplicates":"Select this option if the responses should be averaged over replicates. See <a href=\"https://www.quantics.co.uk/blog/understanding-true-replicates-and-pseudo-replicates-in-bioassay/\" target=\"_blank\">our blog post</a> for more information.","warning-analysisdef-lackoffit":"DO NOT USE Lack-of-fit for estimating variance except for compatibility with some other software. <br />It creates inappropriate confidence intervals. See <a href=\"https://www.quantics.co.uk/blog/when-is-pure-error-for-bioassay-an-error/\" target=\"_blank\">our blog post</a> for more information.","warning-analysisdef-pureerror":"DO NOT USE Pure error for estimating variance except for compatibility with some other software. <br />It creates inappropriate confidence intervals. See <a href=\"https://www.quantics.co.uk/blog/when-is-pure-error-for-bioassay-an-error/\" target=\"_blank\">our blog post</a> for more information.","warning-analysisdef-rsquared":"R<sup>2</sup> is available for bridging purposes. This is not a good measure of goodness of fit. See our <a href=\"https://www.quantics.co.uk/blog/r-we-squared-yet-why-r-squared-is-a-poor-metric-for-goodness-of-fit/\" target=\"_blank\">blog post for a more detailed explanation."};